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Colony forming unit (CFU) assay is extensively used as a medium to study the capability of HSC to proliferate and differentiate by their ability to form progenitor colonies in methylcellulose semisolid media. For a standard transplant procedure, approximately 1–3 × 10 7 cell per kg of recipient weight is needed, whereby a higher dose of infused NCC and CD34+ cells will result in a shorter period of engraftment. However, the success rate of HSC transplant depends on the number and engraftment of infused nucleated cell count (NCC) and CD34+ cells. Additionally, it is easier to collect UCB since it poses less risk to the donor (pregnant mother), has less risk of infection, is accessible, and most importantly the loose criteria for Human Leucocyte Antigen typing match with less graft versus host disease. This may be due to the ability of the HSC pool within UCB to proliferate and differentiate more rapidly, being superior to HSC extracted from bone marrow and peripheral blood. Approximately 30,000 transplantations using UCB, either from a family-related donor or non-related donor, have been reported worldwide. Umbilical cord blood (UCB) has been widely used as an alternative to haematopoietic stem cell (HSC) in transplantation for both malignant and non-malignant diseases including metabolic diseases.

These findings should be taken into consideration when selecting UCB from preeclamptic mothers for banking and predicting successful treatment related to UCB transplant. In addition, the UCB-enriched CD34+ population were lymphoid progenitors and capable to differentiate into natural killer cells and T-lymphocytes. This correlates with the low UCB volume, TNC and CD34+ cells count. The HSC progenitor’s colonies from the preeclampsia group were significantly lower compared to the control. A panel of HSC markers including differentiated haematopoietic markers were used to confirm the differentiation ability of UCB-HSC by flow cytometry analysis. Mononuclear cells (MNC) were isolated from UCB and further enriched for CD34+ cells using immune-magnetic method followed by CFU assay.

The aim of this study is to investigate the effects of preeclampsia in pregnancy on the stemness and differentiation potency of UCB-HSC. Colony forming unit (CFU) assay is widely used as an indicator to predict the success of engraftment, since direct quantitative assay for HSC proliferation is unavailable. Total nucleated cell count (TNC) is an important parameter that can be used to predict engraftment including UCB banking. However, few studies have shown that a common disease in pregnancy such as preeclampsia would affect the quality of UCB-HSC. Umbilical cord blood (UCB) has been proposed as the potential source of haematopoietic stem cells (HSC) for allogeneic transplantation.

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